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Chinese Scientists Use In Vitro Multienzyme System To Achieve Complete Conversion Of Cellulose
- Nov 02, 2018 -

Recently, tianjin institute of industrial biotechnology, Chinese academy of sciences swim chun researcher team in ACS catalysis magazine published online (IF = 11.384) the titled "Stoichiometric Conversion of Cellulosic Biomass by in Vitro Synthetic Enzymatic Biosystems for Biomanufacturing" research paper.In this study, the method of in vitro synthetic biology was used to realize the biotransformation of cellulose stoichiometry for the first time, which provided a new research idea for cellulose conversion and synthesis of high-value bio-based chemicals.

Cellulose is the most abundant renewable resource on the earth and an important raw material for the production of bio-fuels and bio-based chemicals.In vitro enzyme system was more mimic in vivo metabolic pathway, the combination of a series of enzymes in vitro and coenzyme building complex biochemical reaction network, catalysis substrates to generate new green biological manufacturing platform, has been successfully applied to the use of starch production of hydrogen, bioelectricity, rare sugar and other biological manufacturing process, but to use the system efficiently convert cellulose synthesis of high value-added chemicals is not yet implemented.

Against the international problem, tianjin institute of industrial biotechnology, Chinese academy of sciences by means of synthetic biology, in vitro construction of three phosphorylase cascade way, the fiber polysaccharides into high quality phosphorylated sugar, under the action of enzyme catalytic conversion of downstream synthetic inositol, inositol production rate reached 98%, close to stoichiometric substrate conversion rate, and the multiple enzyme system in vitro can directly use of high toxic hydrolyzed corn straw as a substrate synthesis of inositol.This research provides a new idea for the high value utilization of cellulose.